Figure 3

Deficiency in Slc1a4 does not affect development of M-cells and motor function of juvenile fish. (a) Hybridization of transgenic line: Tg (Tol 056: EGFP) and slc1a4 mutants were crossed for two consecutive generations to obtain Tg (Tol 056: EGFP)/slc1a4^+/− and Tg (T056: EGFP); slc1a4^−/− lines. (b) Representative images of embryos from wild type and mutant at 6 dpf (scale bar, 500 μm). (c) Representative images of area of cell body (scale bar, 50 μm). (d) Representative images of relative axon length from cloacal pore to end (scale bar, 50 μm). Asterisk, ablation site. (e) Statistical quantitative diagram represents length of fish at 6 dpf. Data shown as mean ± sem (WT/AB: 4.1 ± 0.1 mm, n = 9; slc1a4^−/−: 4.2 ± 0.0 mm, n = 10). Assessed by unpaired t-test. ns, not significant. (f) Statistical quantitative diagram represents area of cell body. Data shown as mean ± sem (WT/AB: 399.3 ± 5.9 μm², n = 6; slc1a4^−/−: 399.7 ± 3.9 μm², n = 6). Assessed by unpaired t-test. ns, not significant. (g) Statistical quantitative diagram represents relative axon length. Data shown as mean ± sem (WT/AB: 1498.0 ± 6.1 μm, n = 6; slc1a4^−/−: 1485.0 ± 3.0 μm, n = 6). Assessed by unpaired t-test. ns, not significant. (h,i) Line illustrates 6 dpf zebrafish larvae’s swimming trajectory differences from WT and slc1a4^−/− groups evaluated over 1 h. Data shown as mean ± sem (WT/AB: 1230.0 ± 70.1 cm/h, n = 6; slc1a4^−/−: 844.2 ± 58.0 cm/h, n = 12). Assessed by unpaired t-test. ns, not significant.