FIGURE

Fig. 2

ID

ZDB-FIG-241015-32

Publication

Melikishvili et al., 2024 - Effectiveness of add-on acetazolamide in children with drug-resistant CHD2-related epilepsy and in a zebrafish CHD2 model

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Fig. 2

Antisense knockdown of chd2 in zebrafish larvae?morphological and electrophysiological analysis (A) chd2 knockdown and non-injected control zebrafish larvae at 5 dpf. (B-C) Local field potential (LFP) recording revealed epileptiform events in 5-dpf chd2 knockdown larvae; chd2 knockdown larvae exhibited an increase in ictal-like events compared to non-injected control larvae at 5 dpf, non-injected control larvae with a mean of 0.29 � 0.18 (SEM), chd2 knockdown larvae with a mean of 3.29 � 0.77, chd2 knockdown treated with 1 mM acetazolamide with a mean of 0.92 � 0.29, chd2 knockdown treated with 100 ?M fenfluramine overnight with a mean of 2.0 � 0.64 ictal-like events. Statistical analysis by one-way ANOVA showed that treatment with 1 mM acetazolamide significantly reduced ictal-like events (p < 0.05), but that for 100 ?M fenfluramine, the result was not significant (p > 0.29), with all groups passing the D'Agostino-Pearson normality test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-chd2
Conditions:	chemical treatment by environment: acetazolamide chemical treatment by environment: fenfluramine
Knockdown Reagent:	MO1-chd2
Observed In:	action potential initiation cranial skeletal system development head pectoral fin development pericardium post-vent region swim bladder
Stage:	Day 5

Phenotype Detail

Acknowledgments

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