FIGURE

Fig. 4

ID

ZDB-FIG-241009-12

Publication

Ugur et al., 2024 - VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1

Other Figures

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Fig. 4

A partnership of fam177a1 and vps13b in zebrafish. (A) Alphafold2 prediction of human VPS13B and zebrafish Vps13b. (B) Representative images of WT and fam177a1a;fam177a1b DKO (fam177a1DKO) zebrafish embryos that are either uninjected or injected with vps13b gRNAs/Cas9. The left panel shows embryos treated with DMSO at 6 hpf until 25 hpf whereas right panel shows embryos treated with 0.8 µg/ml BFA at 6 hpf until 25 hpf. (C) Quantifications of tail length results shown in B. Data are shown as means ± SEM, Unpaired, two-tailed t tests. n.s., not significant. ****P < 0.0001; ***P < 0.001; **P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB AB + CRISPR1-vps13b + CRISPR2-vps13b fam177a1^{stl700/stl700}; zgc:153383^{stl746/stl746} (AB) fam177a1^{stl700/stl700}; zgc:153383^{stl746/stl746} + CRISPR1-vps13b + CRISPR2-vps13b (AB)
Condition:	chemical treatment by environment: brefeldin A
Knockdown Reagents:	CRISPR1-vps13b CRISPR2-vps13b
Observed In:	post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.