Fig. 2

Wgx-50 inhibits the recruitment of neutrophils and the expression of pro-inflammatory cytokines induced by DSS. (A) Images display the chemical formula of Wgx-50. (B) Images demonstrate the recruitment of neutrophils in the zebrafish intestinal injury model under fluorescence microscopy. White rectangles represent counting areas. Scale: 100 μm. (C) The statistical quantification of neutrophil recruitment in zebrafish intestinal injury model under control group, DSS group, and DSS + Wgx-50 group. The recruitment of neutrophils in the zebrafish intestine increases after DSS treatment, and the number of neutrophil recruitment gradually decreases with the addition of Wgx-50 over time. (D–G) The gene expression levels of pro-inflammatory cytokines il-1, il-6, cxcl8a, and tnf-α in zebrafish under control group, DSS group, and DSS + Wgx-50 group. The levels of pro-inflammatory cytokines increase after DSS treatment, and the levels gradually decrease with the addition of Wgx-50 over time. The primer sequences are listed in Table 1. (ns, not significant;, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one-way ANOVA.)