FIGURE

Fig. 6

ID

ZDB-FIG-240904-25

Publication

Ma et al., 2024 - Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase

Other Figures

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Fig. 6

ubi:EGFPtyr transgenic fish exhibit robust and ubiquitous EGFP expression. (A) Bright-field and fluorescence images of ubi:EGFPtyr embryos versus wide-type (WT) at various developmental stages as indicated. Each panel contains an ubi:EGFPtyr embryo on the left and a WT embryo on the right. hpf, hours post fertilization; dpf, days post fertilization. (B) Histology sections of ubi:EGFPtyr adult fish stained with H&E or anti-EGFP antibody (IHC) demonstrating widespread but varying levels of EGFP expression among different tissue and cell types. All images were acquired under 400X magnification. (C) Representative flow cytometry analysis of the wild-type and ubi:EGFPtyr adult whole kidney marrow (WKM). Major blood lineages in WKM were resolved and identified as previously shown (left panels).36 EGFP expression was analyzed in each blood cell gate (middle and right panels). Gray, wild-type; pink, ubi:EGFPtyr.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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