FIGURE

Figure 6

ID

ZDB-FIG-240902-15

Publication

Marques et al., 2024 - Activation of Nrf2 at Critical Windows of Development Alters Tissue-Specific Protein S-Glutathionylation in the Zebrafish (Danio rerio) Embryo

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Figure 6

Zebrafish were treated with 40 µM SFN or 1 µM tBHQ for 6 h during the pharyngula, hatching, and protruding-mouth stages and incubated with BioGee for 2 h before fixation 24 h after the start of the exposure. BioGee protein conjugates were labeled in situ using IHC. Confocal microscopy was used to acquire Z-stacks of the islet. (A) Islet volume and (B) islet mean fluorescence intensity (FI) of BioGee. Representative images at the (C) pharyngula (start of exposure)/hatching (BioGee labeling and fixation), (D) hatching/protruding-mouth, and (E) protruding-mouth/larval stages are shown. Images are max intensity projections of the Z-stack the islet (outlined in yellow), where FITC (green) represents β-cells, TRITC (purple) represents BioGee protein conjugates, and DAPI (blue) represents nuclei. Statistical significance was assessed via two-way ANOVA followed by Fisher’s LSD post hoc test. n = 7–9 fish. Letters indicate significant differences (p ≤ 0.05) among groups. Scale bar = 25 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)