FIGURE

Fig. 5

ID

ZDB-FIG-240828-5

Publication

Duara et al., 2024 - Oxysterol-binding protein like 7 deficiency leads to ER stress mediated apoptosis in podocytes and proteinuria

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Fig. 5

Proteinuria and glomerular damage in zebrafish with Osbpl7 knockdown. Osbpl7 knockdown by osbpl7-Mo in l-fabp:DBP-eGFP ZF led to decreased eye fluorescence (A) and increased edema (B). C: representative images of individual zebrafish. D: transmission electron microscopy (TEM) images of the zebrafish glomerulus at 5 DPI with either Control-Mo and osbpl7-Mo. Substantial glomerular damage was conspicuous in the osbpl7-Mo-injected larvae. The detailed TEM analysis revealed the loss of endothelial fenestrations (white arrows) and widening and rupture of the glomerular basement membrane (GBM) (star). The distinct alterations in podocyte structures are shown, including podocyte effacement and protrusions of foot processes into the GBM (black arrows), a common hallmark of proteinuric states in fish. These observations point to clear podocyte and glomerular damage, in alignment with the proteinuria documented in the fish. 4 DPI. n = 16. **P < 0.01 and ****P < 0.001. OSBPL7, oxysterol-binding protein-like 7.

Expression Data

Gene:	EGFP
Fish:	li1Tg lri500Tg li1Tg + MO1-osbpl7 li1Tg + MO2-osbpl7 lri500Tg + MO1-osbpl7 lri500Tg + MO2-osbpl7
Knockdown Reagents:	MO1-osbpl7 MO2-osbpl7
Anatomical Terms:	eye pronephros
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lri500Tg + MO1-osbpl7 lri500Tg + MO2-osbpl7
Knockdown Reagents:	MO1-osbpl7 MO2-osbpl7
Observed In:	eye glomerular basement membrane glomerular filtration pericardium podocyte pronephric glomerulus
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Renal Physiol.