FIGURE

Figure 5

ID

ZDB-FIG-240823-33

Publication

Grepper et al., 2024 - BCL2L13 at endoplasmic reticulum-mitochondria contact sites regulates calcium homeostasis to maintain skeletal muscle function

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Figure 5

Subcellular localization of BCL2L13 and ERMCs quantifications

(A) Representative western blot of isolated subcellular fractions. Expression of BCL2L13, FACL4, NDUFS3, and SERCA2 in crude mitochondria (CM), pure mitochondria (PM), EMRCs, ER, and cytosol (Cyt). Experiments were repeated 4 times.

(B) Representative confocal microscopy images of HeLa cells expressing SPLICS_S/L probes (green) and BFP from P2A constructs (blue).

(C) Quantification of SPLICS_S and (D) SPLICS_L contacts in HeLa cells expressing control-P2A-BFP (Control) or BCL2L13-P2A-BFP (BCL2L13). n = 30 cells from four independent transfections.

(E) Number of ERMCs per mitochondria and (F) percentage of the mitochondrial membrane in contact with ERMCs (% mito mb in contact) measured in electron micrographs from siControl and siBCL2L13 transfected C2C12 myotubes.

(G) Representative electron micrographs of C2C12 myotubes in siControl and siBCL2L13 transfected cells. Orange arrows indicate examples of ERMCs. n = 27 micrographs (4.95 × 4.95 μm) per condition from four independent experiments. For all panels, the median is shown by a white full line while quartiles are dotted lines.