Fig. 5
Effect of Dextrose, CUMS, and CUMS + Dextrose treatment on total, cytosolic and nuclear NRF2 protein content. NRF2 representative western blots (a), total NRF2 relative protein content (b), cytosolic relative NRF2 protein content (c), nuclear relative NRF2 protein content and relative mRNA quantification of indicated genes: nrf2 (e), and keap1 (f). Nonparametric test was used for statistical analysis of mRNA content of nrf2 and keap1. Data presented is the average of three determinations of different homogenates obtained from three-five pooled zebrafish brains. (*, p < 0.05; **, p < 0.01; ***, p < 0.001) ****, p < 0.0001). Each value is represented as mean ± SEM. (n = 3). |