Fig. 5

Emergence of an injury-responsive iNeuron signature during spinal cord regeneration. A Dynamics of neuronal subclusters at 0, 1, 3 and 6 wpi. Cell proportions for each cluster and time point were normalized to the total number of neurons analyzed at that time point. Clusters that either increased or decreased by >2-fold in proportion are highlighted in red and blue, respectively. B UMAP plot showing the predicted cluster identity for each neuron subpopulations. V0, V2a, V2b, interneurons, motor neurons are shown along with clusters identified as neuroblast-like or progenitor-like neurons. Cluster N20 was the only cluster identified as neuroblast-like neurons. C UMAP plot showing a calculated ?Regeneration Score? for each neuron subclusters. -log10(p value) of each cluster is represented in a gradient scale of gray (min = 0) to red (max = 6). Cluster N20 showed the highest regeneration score compared to all other neurons. D Feature plots show the expression of the regeneration associated genes gap43 and atf3 in cluster N20. E Gene ontology of N20 iNeuron DE markers. Twenty of the most enriched terms are shown. X-axis represents ?log10(p value) for each term. F Circle plots represent cell-cell interaction strengths at 1 wpi. Arrow thickness is directly proportional to the cumulative strengths of all predicted interactions between clusters. G Signaling pathways outgoing from or received by iNeurons at 1 wpi. Bar graphs at the top of each heatmap show cumulative signaling strengths per cell population. Bar graphs at the right of each heatmap show cumulative signaling strength per pathway. One-tailed hypergeometric test is performed in (C, E). Source data are provided as a Source Data file.