Fig. 4

Absent regeneration of cone visual pigments in Zcrdh-mutant retinal explants following a photobleach and treatment with 11cROL chromophore precursor Absorbance spectra of wild-type and Zcrdh-mutant zebrafish photoreceptors. (A) Average absorbance spectra from wild-type zebrafish M cones. The mean peak optical densities (± SEM) were ODDA = 0.024 ± 0.0025, ODbleach = 0.0036 ± 0.0033, and OD11cROL = 0.018 ± 0.0043. (B) Average absorbance spectra from wild-type zebrafish L cones. The mean peak optical densities were ODDA = 0.030 ± 0.0043, ODbleach = 0.0091 ± 0.0041, and OD11cROL = 0.021 ± 0.0039. (C) Average absorbance spectra from wild-type zebrafish rods. The mean peak optical densities were ODDA = 0.044 ± 0.0050, ODbleach = 0.0076 ± 0.0031, and OD11cROL = 0.0060 ± 0.0019. (D) Average absorbance spectra from Zcrdh-mutant zebrafish M cones. The mean peak optical densities were ODDA = 0.021 ± 0.0040, ODbleach = 0.0023 ± 0.0099, OD11cROL = 0.0059 ± 0.0038, and OD11cRAL = 0.017 ± 0.0028. (E) Average absorbance spectra from Zcrdh-mutant zebrafish L cones. The mean peak optical densities were ODDA = 0.018 ± 0.0037, ODbleach = 0.0033 ± 0.0070, OD11cROL = 0.0052 ± 0.0035, and OD11cRAL = 0.021 ± 0.0017. (F) Average absorbance spectra from Zcrdh-mutant zebrafish rods. The mean peak optical densities were ODDA = 0.029 ± 0.0028, ODbleach = 0.0020 ± 0.0028, OD11cROL = 0.0050 ± 0.0024, and OD11cRAL = 0.023 ± 0.0020. Data from dark-adapted and regenerated cells are fitted with A1 nomograms with the following parameters: WT DA M-cone, λmax = 492 nm, ODpeak = 0.025; WT 11cROL M-cone, λmax = 500 nm, ODpeak = 0.017; WT DA L-cone, λmax = 555 nm, ODpeak = 0.033; WT 11cROL L-cone, λmax = 547 nm, ODpeak = 0.024; WT DA rod, λmax = 498 nm, ODpeak = 0.047; Zcrdh-mutant DA M-cone, λmax = 502 nm, ODpeak = 0.018; Zcrdh-mutant 11cRAL M-cone, λmax = 491 nm, ODpeak = 0.021; Zcrdh-mutant DA L-cone, λmax = 554 nm, ODpeak = 0.022; Zcrdh-mutant 11cRAL L-cone, λmax = 551 nm, ODpeak = 0.023; Zcrdh-mutant DA rod, λmax = 507 nm, ODpeak = 0.027; Zcrdh-mutant 11cRAL rod, λmax = 510 nm, ODpeak = 0.022.