Fig. 5
- ID
- ZDB-FIG-240802-68
- Publication
- Zhao et al., 2024 - Artemvulactone E isolated from Artemisia vulgaris L. ameliorates lipopolysaccharide-induced inflammation in both RAW264.7 and zebrafish model
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AE inhibits the activation of NF-?B. (A) RAW264.7 were treated with LPS and AE for 12 h, and Western blot was then performed. (B) Relative protein expression levels of p-IKK?/?, p-p65 and p-I?B?. (C) The cells were treated with LPS and AEfor 12 h, NF-?B p65 was immunostained with anti-p65 antibody and detected using secondary antibody conjugated with Alexa FluorTM 488 (green) followed by DAPI (blue) staining for nuclei. Scale bar, 20 ?m. Data were represented as mean ± SEM (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001;****p < 0.0001; ns, no significance, compared with each?group. ##p < 0.01; ###p < 0.001; ####p < 0.0001; compared with Ctrl group. |