Fig. 2
- ID
- ZDB-FIG-240724-7
- Publication
- Cotti et al., 2024 - Matrix first, minerals later: fine-tuned dietary phosphate increases bone formation in zebrafish
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Expression of osteocalcin in the vertebral column assessed in transgenic osc:GFP zebrafish line fed LP, RP, HP for two months. (A–C) Independent from dietary P intake, osteocalcin is expressed by osteoblasts at vertebral body endplates (yellow arrowhead, A) and by bone lining cells at the bone surface (white arrows, A,B), the trabecular bone (magenta arrows, A) and arches (blue arrows, C). Dashed lines indicate the inner bone surface. Osteocytes (white arrowheads in B and C) are osc:GFP positive. The osteocytic canalicular network is also labeled (yellow arrows, C). (D) Toluidine blue stained zebrafish vertebral endplates (i) showing osteoblasts (yellow arrowheads), fibroblasts (magenta arrowheads), and osteocyte (white arrowhead). Osteoblasts (yellow arrowheads) from LP (ii), RP (iii), and HP (iv) zebrafish are osc:GFP positive, but fibroblasts (magenta arrowheads) are osc:GFP negative. (E) Selection of the area used for quantification of the osc:GFP-positive area occupied by osteoblasts at vertebral body endplates, considering only osteoblasts and excluding osteocytes (white arrowhead) and bone lining cells (light blue arrowheads). (F) LP zebrafish have a significantly increased osc:GFP signal area, i.e. the area occupied by GFP-positive osteoblasts, at vertebral endplates compared to RP and HP. Values are reported with Box and Whisker plots: the midline in each box is indicative of the median, whereas min and max values are shown with whiskers. Individual data points and P values are shown. |