FBLN5 is required for myelination of Schwann cells. A, A diagram of zebrafish larvae identifying the simplified peripheral nervous system, consisting of the spinal cord tracts, lateral lines, and the spinal motor nerves. A, anterior; P, posterior. B, Lateral view images of the whole-mounted Tg(claudin K:gal4-vp16;uas:egfp) zebrafish injected with control-, fbln5-MOs, or fbln5 mRNA after DAPI staining at 4 dpf. PLLn (green), posterior lateral line; A, anterior; P, posterior. OE, overexpression. Scale bars, 20 μm. C, Quantification of cell numbers counted within the PLLn of (B) images. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test (*P < .05, **P < .005). D, Lateral view images of the Tg(claudin K:gal4-vp16;uas:egfp) zebrafish injected with control-MOs or fbln5-MOs at 5 dpf. The images within the rectangles are magnified in the bottom panels. SCT, spinal cord tracts; PLLn, posterior lateral line; A, anterior; P, posterior. Scale bars, 100 μm. E, Quantification of the relative intensity of PLLn in equivalent fields of view in the images of (D). Statistical significance was determined using the unpaired Student's t-test with Welch's correction (***P < .001). The data are shown as the mean ± SD of three independent experiments with ≥20 embryos per condition (C and E)
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