Fig. 6

Clonidine enhances sleep, and rebound sleep, in dbh F0 KO larvae. a Sleep traces for dbh F0 KO and control-injected larvae exposed to clonidine/DMSO and caffeine. b At 5 dpf from clonidine treatment until lights-out, clonidine had a positive effect on sleep levels in both control-injected and dbh F0 KO larvae, as illustrated by positive effect sizes and 95% CIs. The effect size of clonidine was greater among control-injected larvae. The post-caffeine rebound sleep period is summarised for each larva in c. Among dbh F0 KOs, clonidine had a positive effect on rebound sleep. The clonidine effect size was not significantly different between the two genotypes. Deep sequencing was used to verify the successful loss-of-function targeting of dbh in 10 randomly selected dbh F0 KO larvae: all animals had > 93% (mean, 96%) of their amplified dbh copies frameshifted (see Fig. S10)