Fig. 6

NO signaling mediates the effects of BF170 on hematopoietic stem and progenitor cell induction. (A) Increased expression of the NO sensor klf2a in BF170-treated embryos. Outlined area indicates aorta-gonad-mesonephros. Box and whisker plots show the quantification of positive signals in outlined area. (B) BF170-induced increased expression of the NO signaling genes nos1 and nos2b in flk1+cmyb+ double-positive cells. (C,D) The NO inhibitor L-NAME blocked BF170-induced hematopoietic stem and progenitor cell (HSPC) emergence in the embryos. Red arrowheads indicate positive signals. Box and whisker plots in C and D show quantification of cmyb and flk1 expression in embryos from different groups. Images are lateral views with anterior to the left and dorsal upwards. In A,C,D, Nchanged/Ntotal in the bottom right corner of each image indicates embryos with changed expression/total tested embryos. n values indicate the number of embryos with changed expression in each group. Each experiment was repeated three times. Data are mean±s.d. In the box and whisker plots, the boxes indicate mean and the error bars indicate s.d. Whiskers indicate min to max values and all data points are shown. **P<0.01, ***P<0.001. Scale bars: 100 μm.