Fig. 8

The protective effect and mechanism of QXY on ISO-induced cytoskeleton reorganization and hypertrophy in H9c2 cells. (A) The H9c2 cells were treated with ISO (100 µM), with or without QXY (3 µg/ml, 5 µg/ml), for 24 h. The intracellular cytoskeleton and nuclei were stained with Actin-Tracker Red-Rhodamine and DAPI, respectively. The protein concentration of cells was measured by BCA analysis. (B-C) The H9c2 cells were treated with ISO (100 µM), with or without QXY (5 µg/ml), for 24 h. The protein expression levels of phosphor-SMAD2/3, SMAD2, α-SMA, Vimentin, RhoA, ROCK1, phosphor-MYPT1, MYPT1, phosphor-MLC, and MLC were detected by western blotting. GAPDH served as the internal control. All the data were presented as mean ± SEM. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs control (Ctrl) group; *p < 0.05 and **p < 0.01 vs ISO-treated group. n = 4–6.