Fig. 7

The protective effect of QXY on ISO-induced calcium influx and mitochondrial dysfunction in H9c2 cells. (A) The transcriptome profile of the regulation of gene expression and its GO enrichment of differentially expressed genes (DEGs) between QXY-treated and control groups. Red rectangle presented that DEGs were enriched with the item of calcium binding. (B) The intracellular calcium imaging of H9c2 cells, which were pre-treated with QXY (20 µg/ml) or nifedipine (NIF, 2 µM) for 15 min, challenged with ISO (1 µM). The L-type calcium channel blocker NIF served as the positive control. The intracellular Ca2+ change was presented as the real-time fluorescence intensity (F1) to the fluorescence intensity before stimulation (F0), which indicated by the color change in the real-time imaging. (C-D) The H9c2 cells were treated with ISO (100 µM), with or without QXY (3 µg/ml, 5 µg/ml), for 24 h. The mitochondrial membrane potential were detected by JC-1 staining and calculated by the fluorescence intensity ratio of Green (monomeric JC-1) / Red (aggregated JC-1). (E-F) The H9c2 cells were treated with ISO (100 µM), with or without QXY (5 µg/ml), for 24 h. Then the cells were harvested and followed by intracellular MDA and ATP assays according to the manufactures. All the data were presented as mean ± SEM. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs control (Ctrl) group; *p < 0.05 and **p < 0.01 vs ISO-treated group. n = 4–6.