Assessment of the response ability and locomotor behavior in the light-dark test. A: Locomotor patterns and average speed during dark-light stimulations of zebrafish after exposure to four different combinations of AlCl3 and D-gal from 2 to 5 dpf. B: Quantitative analysis of the average speed change during light-dark stimulations. C: Representative traces of total locomotor activity in different groups during all light-dark stimulations. D: Quantitative analysis of total distance swam during all light-dark stimulations. In B and D, data are presented as the mean ± SEM (n = 10 zebrafish larvae per group). Statistical significance of treatment effects was assessed using one-way ANOVA with Dunnett’s multiple comparison test and illustrated as follows: * * p < 0.01 (versus the control group).
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