FIGURE

Figure 5

ID

ZDB-FIG-240607-124

Publication

Fabian et al., 2024 - Comprehensive phenotypic characterization of an allelic series of zebrafish models of NEB-related nemaline myopathy

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Figure 5

Muscle and sarcomere organization is defective in neb mutants. (A) Confocal micrographs of wholemount preparations of embryos at 6 dpf stained with antibody against α-Actinin and Rhodamine Phalloidin. Nonsense mutants and the splice mutant neb²¹ show disorganized, misaligned or broken muscle fibers (arrows), defects in the MTJ regions, with partially detached fibers and accumulations of aggregates of actin filaments (arrowheads), and aggregates of actin/α-Actinin (open arrowheads). Scale bar = 20 μm. (B) Transmission electron micrographs of zebrafish embryos at 6 dpf showing defects in the MTJ regions (dotted line), with disorganized muscle fibers (arrows) and misaligned sarcomeres (arrowheads) in the nonsense, neb³⁴, and neb²¹ splice mutants. Scale bars = 2 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	neb^{sa11973/sa11973} neb^{sa15371/sa15371} neb^{sa21475/sa21475} neb^{sa30646/sa30646} neb^{sa34628/sa34628}
Observed In:	skeletal muscle cell skeletal muscle cell actin filament skeletal muscle cell muscle tendon junction skeletal muscle cell sarcomere skeletal muscle cell skeletal muscle myofibril skeletal muscle cell Z disc
Stage:	Day 6

Phenotype Detail

Acknowledgments

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