Figure 6—figure supplement 1.

Styxl2 regulates the protein levels of NM IIs without affecting their mRNA.

(A) Control (Ctrl) or Styxl2 DNA constructs were transfected into C2C12 cells. 24 hr later, the mRNA levels of different genes were determined by qPCR. **p-value <0.01. n.s.: not significant. (B) Zebrafish zygotes were injected with various morpholinos as indicated with or without the co-injected Styxl2 mRNAs. zStyxl2FL: the full-length fish Styxl2 mRNA; zStyxl2ΔN493: the truncated fish Styxl2 mRNA missing the 5’ region encoding the N-terminal 493 aa. Representative immunofluorescent images of myosin heavy chain (MHC) were shown. The number in numerator at the top right corner of each image represents fish embryos showing normal MHC staining pattern, while that in denominator represents the total number of embryos examined. Scale bar: 10 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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