Figure 2—figure supplement 1.
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- ZDB-FIG-240603-100
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- Spikol et al., 2024 - Genetically defined nucleus incertus neurons differ in connectivity and function
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Transgenic driver lines recapitulate gsc2 and rln3a expression patterns. (A, D) CRISPR/Cas9 genome editing strategies used to generate (A) Tg(gsc2:QF2)c721 and (D) Tg(rln3a:QF2, he1.1:YFP)c836 driver lines. (B, C, E, F) Dorsal views of 6 dpf larvae, anterior to the top. (B, E) WISH for (B) gsc2 and (E) rln3a. (C, F) Confocal Z-projections of (C) Tg(gsc2:QF2)c721; Tg(QUAS:GFP)c578 and (F) Tg(rln3a:QF2, he1.1:YFP)c836; Tg(QUAS:GFP)c578 larvae. Scale bars, 100 µm. sgRNA: single guide RNA, hsp70: heat shock cognate 70-kd protein, tandem duplicate 1 promoter, 5’ UTR: 5’ untranslated region, HA: homology arm, he1.1: promoter of hatching enzyme gene. |