Fig 3

Naturally transmitted RMBV infections induce systemic inflammatory antiviral responses in laboratory zebrafish.

(A) Differential gene expression in laboratory zebrafish infected by RMBV compared to uninfected laboratory zebrafish. Average log2 fold change expression is plotted for each gene in a comparison of 3 RMBV-infected and 3 uninfected samples for each tissue. All comparisons had adjusted p-values of <0.05 unless denoted with a black circle. (B) Comparison of RMBV-induced gene sets by tissue. Euler plot includes all genes with log2 fold changes >1 and adjusted p-values <0.05. (C) Comparison of virus-induced gene sets in intestine, spleen, or kidney during RMBV or SVCV infections. Euler plot includes all genes with log2 fold changes >2 and adjusted p-values <0.05. SVCV RNAseq data are from (PMID: 34908463). (D) Enrichment of Gene Ontology biological processes among the genes that are induced with log2 fold changes >2 during RMBV or SVCV infections. All biological processes with false discovery rates of <0.05 for at least 1 condition are plotted. RMBV, Rocky Mountain birnavirus; SVCV, spring viremia of carp virus.