Fig. 5

Enhanced autophagy in the liver of larsb-knockin larvae. A Immunostaining of LC-3 in the liver of larsb-knockin (larsb-I451F) larvae at 5 dpf. Scale bar: 20 μm. White arrowheads indicate LC3-positive dots. B Quantification of the number of LC-3 dots in larsb-WT (n = 10 sections) and larsb-I451F (n = 9 sections) larvae liver at 5 dpf. Error bars indicate SEM. **P < 0.01. C Morphological abnormality in the livers of larsb-I451F larvae at 5 dpf with a Tg[fabp10:mcherry] background treated with DMSO or bafilomycin. Scale bar: 200 μm. D Liver area in larsb-I451F larvae at 5 dpf with a Tg[fabp10:mcherry] background treated with DMSO (larsb-WT n = 11 sections, larsb-I451F n = 9 sections) or bafilomycin (larsb-WT n = 10 sections, larsb-I451F n = 14 sections). Error bars indicate SEM. *P < 0.05. E Circularity of the liver in larsb-I451F larvae at 5 dpf with a Tg[fabp10:mcherry] background treated with DMSO (larsb-WT n = 11 sections, larsb-I451F n = 9 sections) or bafilomycin (larsb-WT n = 10 sections, larsb-I451F n = 14 sections). Error bars indicate SEM. *P < 0.05. F Lipids staining of the liver in larsb-I451F larvae at 5 dpf treated with DMSO or bafilomycin. Scale bar: 20 μm. G Quantification of the lipid area in larsb-I451F larvae liver at 5 dpf treated with DMSO (larsb-WT n = 10 sections, larsb-I451F n = 11 sections) or bafilomycin (larsb-WT n = 14 sections, larsb-I451F n = 9 sections). Error bars indicate SEM. **P < 0.01. Dpf: days post fertilization