Figure 3

Whole mount immune fluorescence staining cleaved caspase-3. Vybrant CM-DiI labeled LGSOC cells (red), nuclei stained with DAPI (blue) and anti-cleaved caspase-3 (violet). (a–d) The number of xenografts analyzed for cleaved caspase-3 is indicated in the figures; all images are at 25× magnification (scale bar 50 µM). Tumor area is indicated with a white line. (e,f) Quantification of cleaved caspase-3 expression in zebrafish xenografts using Cell Counter plugin in ImageJ. Cells positive for cleaved caspase-3 are indicated by yellow arrows. (g) Ratios of different treatment conditions are presented as AVG ± SEM and each dot represents one xenograft. Multiple comparisons between treatment groups were determined by One-way ANOVA; Tukey procedure was used to adjust p-values. p-values of 0.05 were considered as statistically significantly different and output was represented as: * p ≤ 0.05, *** p ≤ 0.001.