FIGURE

Fig. 7

ID

ZDB-FIG-240513-43

Publication

Yuikawa et al., 2023 - Elongation of the developing spinal cord is driven by Oct4-type transcription factor-mediated regulation of retinoic acid signaling in zebrafish embryos

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Fig. 7

Epistasis analyses of tail bud development regarding regulation by pou5f3 and retinoic acid (RA). (A) Embryos obtained from crosses between an en-pou5f3+/− and wild-type fish were treated with DMSO or DEAB at 14 hpf, and then exposed to heat shock at 17 hpf for en-pou5f3 induction. After heat shock, embryos were immediately subjected to WISH and then genotyped to identify Tg embryos (C–F). (B) Alternatively, embryos injected with pou5f3-ERT2 mRNA were treated with DMSO or RA at 14 hpf, treated with DMSO or 4-OHT at 16 hpf, and then subjected to WISH at 18 hpf (G–J). The expression of tbxta was examined by WISH to assess tail bud development. Up-regulation and down-regulation are marked with solid and open arrowheads, respectively. Percentages of embryos with the shown patterns and total embryo numbers are shown in the right-bottom. WISH, whole-mount in situ hybridization. Scale bars, 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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