FIGURE

Fig 5

ID

ZDB-FIG-240511-5

Publication

Ahuja et al., 2024 - The development of brain pericytes requires expression of the transcription factor nkx3.1 in intermediate precursors

Other Figures

All Figure Page

Back to All Figure Page

Fig 5

Reexpression of Cxcl12b increases pericyte numbers in nkx3.1^−/−.

All embryos were imaged dorsally in the head region. In comparison to a nkx3.1^−/− mutant without a Gal4 driver (A), expressing UAS:cxcl12b under the nkx3.1 Gal4 driver TgBAC(nkx3.1:Gal4) increases pericyte numbers (B, C) and pericyte density (D) in nkx3.1 mutants at 75 hpf (n = 21 mutants without nkx3.1Gal4 and 22 with Gal4). In comparison to a nkx3.1^−/− mutant without a Gal4 driver (E), expressing UAS:cxcl12b under the under the pdgfrβ Gal4 driver TgBAC(pdgfrβ:Gal4) does not change pericyte numbers at 75 hpf (F, G; n = 16 mutants without the pdgfrβ:Gal4, and 8 with pdgfrβ:Gal4). Arrowheads mark example brain pericytes (green) labeled by TgBAC(pdgfrβ:GFP). Brain vessels (red) are labeled by Tg(kdrl:mCherry). Statistical significance was calculated using the Student t test. Scale bar is 50 μm. The data underlying this figure can be found in S3 Table.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx3-1^ca116/ca116; ca101Tg; ca504Tg
Observed In:	brain pericyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.