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Fig 5

ID: ZDB-IMAGE-240511-5
Publication: Ahuja et al., 2024 - The development of brain pericytes requires expression of the transcription factor nkx3.1 in intermediate precursors
All Figures: Figures for Ahuja et al., 2024

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Fig 5 Reexpression of Cxcl12b increases pericyte numbers in nkx3.1^−/−.

All embryos were imaged dorsally in the head region. In comparison to a nkx3.1^−/− mutant without a Gal4 driver (A), expressing UAS:cxcl12b under the nkx3.1 Gal4 driver TgBAC(nkx3.1:Gal4) increases pericyte numbers (B, C) and pericyte density (D) in nkx3.1 mutants at 75 hpf (n = 21 mutants without nkx3.1Gal4 and 22 with Gal4). In comparison to a nkx3.1^−/− mutant without a Gal4 driver (E), expressing UAS:cxcl12b under the under the pdgfrβ Gal4 driver TgBAC(pdgfrβ:Gal4) does not change pericyte numbers at 75 hpf (F, G; n = 16 mutants without the pdgfrβ:Gal4, and 8 with pdgfrβ:Gal4). Arrowheads mark example brain pericytes (green) labeled by TgBAC(pdgfrβ:GFP). Brain vessels (red) are labeled by Tg(kdrl:mCherry). Statistical significance was calculated using the Student t test. Scale bar is 50 μm. The data underlying this figure can be found in S3 Table.

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