Fig. 6

FRAP indicates that PGL has high membrane mobility in comparison to TMM. M. marinum were treated with 3-azido pHB or 6-azido trehalose for 18 h, stained with AF488-DBCO, and imbedded in a 1.5% agarose pad. A ROI was drawn around individual cells and bleached with a 488-laser line. (A) Representative images of the fluorescence recovery after photobleaching over time. The scale bar indicates 2 μm. (B) Rates of FRAP. MFI values of photobleached ROIs were normalized by dividing by the total fluorescence intensities of the corresponding whole cells. The plot of these values as a function of time was fitted to a nonlinear regression with a one-phase association. Each symbol represents the average signal from n = 10 cells. (C) Mean mobile fraction determined as the plateau value from the fitted curves, where each point represents the plateau value of an individual cell. Statistical analysis was performed using an unpaired two-tailed t-test represented by ns (not significant) for p > 0.05.