Fig. 5

Metabolic labeling of PGL-producing M. tuberculosis HN878 treated with 3-azido pHB. (A) M. tuberculosis HN878 treated with various concentrations of 3-azido pHB, stained with AF488-DBCO, and analyzed by flow cytometry. Flow cytometry analysis indicates three replicates seeded from different cells. Relative MFI is determined by normalizing against the DMSO control. Statistical analysis was performed using a two-way ANOVA followed by a Dunnett’s multiple comparisons test. Significance is represented by **p < 0.01. (B) Effects of bacterial growth (A600) of M. tuberculosis HN878 treated with various concentrations of 3-azido pHB. The data are representative of three replicates seeded from different cells. Statistical analysis was performed using a two-way ANOVA followed by a Dunnett’s multiple comparisons test. Significance is represented by **p < 0.01 and ****p < 0.0001. (C) PGL-deficient M. tuberculosis strains (Erdman and HN878 Δpks15/1) and PGL-producing M. tuberculosis strain HN878 treated with 200 μM 3-azido pHB, stained with AF488-DBCO, and analyzed by flow cytometry. Flow cytometry analysis indicates three replicates seeded from different cells. Relative MFI is determined by normalizing against the DMSO control. Statistical analysis was performed using an ordinary two-way ANOVA, followed by Šídák’s multiple comparisons test. Significance is represented by ****p < 0.0001 and ns (not significant) for p > 0.05.