FIGURE

Fig. 6.

ID

ZDB-FIG-240412-48

Publication

Carey et al., 2024 - Distinct features of the regenerating heart uncovered through comparative single-cell profiling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6.

Medaka lack primordial myocardium and have few cortical layer cardiomyocytes. (A) RNA in situ hybridization of myl7 labeling myocardium in zebrafish and medaka ventricles of the indicated age. Dotted line indicates border between trabecular and cortical layers. Images are representative of at least three individuals at each time point. Scale bars: 200 µM. (B) UMAP embedding of re-clustered cardiomyocytes identified as either trabecular (tCM) or cortical (cCM). (C) Gene expression dot plot showing expression of marker genes for each CM cell cluster. (D) Proportion of cardiomyocytes in trabecular or cortical cell clusters from all single-cell samples from zebrafish and medaka. (E,F) UMAP embedding of ventricular cardiomyocytes clustered separately from uninjured (E) zebrafish or (F) medaka. (G,H) Gene expression feature plots for top marker genes for primordial cardiomyocytes in zebrafish (E) or medaka (F). (I) RNA in situ hybridization of myl7, acta2, and hey2 in uninjured zebrafish hearts. Scale bars: 200 µM. (J) RNA in situ hybridization of myl7 and acta2 in uninjured medaka heart. Scale bars: 200 µM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open