Fig. 1.

A single-cell atlas of cardiac injury response in zebrafish and medaka. (A) Experimental overview for collection of ventricles and single-cell sequencing. The number of independent samples and total number of quality filtered cells for each time point are indicated. (B) Representative images of Acid-Fuchsin Orange staining of collagen (blue), fibrin (red), and muscle fibers (tan) in heart sections showing cryoinjury-induced fibrin and collagen deposition in both species (arrows). Anatomical labels indicate ventricle (labeled V), atrium (labeled A), and bulbus arteriosus (labeled BA). (C) UMAP embedding of all sampled cells from each species and time point integrated into a single dataset. A total of 22 clusters were identified and colored by major cardiac cell type (cardiomyocyte, orange shades; endothelial/mural, purple shades; epicardial, green shades; leukocyte, blue shades). (D) Gene expression dot plot showing average gene expression of marker genes for cells classified as the indicated cell type. Two marker genes are displayed for each cell type. Dot sizes represent percent of cells expressing the indicated gene (pct.exp), color indicates average scaled gene expression across all cells in the indicated tissue.