Fig. 1

YB‐1 overexpression induces cell cycle alterations, scattering and morphology changes. (A) Cell growth of SPC212 cells stably overexpressing YB‐1 (SPC212^YB1‐s) or the empty vector (SPC212^VC‐s) after 96 h. Data are shown as mean ± SEM of 3 biological replicates performed in triplicate. (B) Cells were subjected to videomicroscopy over 48 h with 10 min intervals. Cell fate maps indicate interphase (gray) and M‐phase duration (black). Cells that died over the 48‐h period are indicated by the bar not reaching the end of the graph. Each bar represents one single cell. (C) M‐phase lengths and (D) doubling times of single cells from videos in (B). Each dot represents one single cell. The horizontal lines indicate the mean. (E) Quantification of colony formation assays. (F) Representative pictures of colony formation assays. Scale bar: 100 μm. (G) Distance to the nearest neighbor of > 160 cells and (H) aspect ratio of > 20 single cells, derived from colony formation assays. Bars show mean ± SEM. Student's T‐test, *P < 0.05, **P < 0.01, ***P < 0.001.