Fig. 10
Effects of estrogen on the expressions of miR-19b-3p and MSMO1. (A) Estrogen downregulates the expression of miR-19b-3p and upregulates the expression of MSMO1 (n = 6). (B) The potential estrogen receptor-binding site located in MSMO1 promoter region. (C) Dual luciferase reporter system verified the estrogen receptor-binding site in MSMO1 (n = 6). (D) Relative expression levels of MSMO1 in LMH treated with different estrogen antagonists (n = 6). WT indicates wild type of the estrogen receptor-binding site, MUT indicates the mutant type of the estrogen receptor-binding site, + indicates added, – indicates not added. |