Figure 3

Effect of CP plus dinaciclib treatment on NT2/D1/-R cells. (a) Cell viability. NT2/D1 (1a) and NT2/D1-R (2a) cells were treated with increasing concentrations of CP alone or in the presence of the corresponding IC₂₅/IC₅₀ dose of dinaciclib (DIN). After forty-eight hours of treatment, cell viability was measured using the MTT assay. Results are expressed as the percentage of viable cells vs. untreated cells (Ctrl) ± SEM. ° p < 0.05 vs. untreated cells, § p < 0.01 vs. untreated cells; # p < 0.001 vs. untreated cells, * p < 0.0001 vs. untreated cells. (b) Cell-cycle analysis. NT2/D1 (1b) and NT2/D1-R cells (2b) were treated with the corresponding IC50 concentration of CP/CP plus dinaciclib for forty-eight hours, and the cell-cycle distribution was analyzed. Histograms representing the percentage of cells in each cell-cycle phase are reported. # p < 0.001 vs. untreated cells, * p < 0.0001 vs. untreated cells.