Fig. 1

FGF19 is highly expressed in NPC. A: Representative results of immunohistochemical staining. The first column: IHC detection of FGF19 in nasopharyngeal epithelium tissues. The second and third columns: IHC detection of FGF19 in NPC tissues of stage I-II. The forth and fifth columns: IHC detection of FGF19 in NPC tissues of stage III-IV (top: × 200, bottom: × 400). B: Western blot analysis of FGF19 expression in 3 NPC tissues and 3 nasopharyngeal epithelium tissues. (T) Nasopharyngeal squamous cell carcinoma tissues. (N) Nasopharyngeal epithelium tissues. Tubulin was used as a control for protein load. C: qRT-PCR was used to detect the relative expression of FGF19 in tissues. D: ELISA was used to detect serum FGF19 levels in 61 NPC patients and 36 healthy volunteers. E: Serum FGF19 levels of NPC patients in stage I- II and stage III- IV. F: Serum FGF19 levels in male and female NPC patients. G: Serum FGF19 levels in NPC patients of different ages. Data are presented as the mean ± SD of three independent assessments. *P < 0.05, **P < 0.01, ***P < 0.001, NS: nonsignificant