Fig. 4

ZAK?/? mice are protected against obesity-associated metabolic dysfunction. (A) Schematic of mouse feeding experiment. Ten- to 12-week-old male WT (n = 13) and ZAK?/? (n = 12) mice were maintained on chow and subjected to MR scanning and ipGTT 2 weeks before shifting to a HFHS diet. Mice were subjected to MR scanning and ipGTT after a further 8 and 19 weeks of HFHS feeding. Mice were euthanized and tissues were collected 25 weeks after the diet shift. (B) Percent body weight gain of mice from (A). Arrows mark the time of IpGTT assay and resulting transient weight loss. (C) Blood glucose concentrations of mice from (A) subjected to ipGTT assay. (D) HOMA-IR of mice from (A). (E) Blood glucose concentration of mice from fig. S4I subjected to the IpITT at the 8-week time point (WT, n = 12; ZAK?/?, n = 7). (F) Liver triglyceride content of mice in (A). (G) Scoring of liver steatosis grade (scale, 0 to 4) of mice from (A). (H) Images of representative hematoxylin & eosin (H&E)?stained WT and ZAK?/? liver sections from mice in (A). Arrows indicate areas of steatosis. For (B) to (F), all data are plotted as mean and all error bars represent the SEM. For (C) and (E), statistical analysis is based on the area under the curve (AUC) for each experimental group. *P ? 0.05; **P ? 0.01 by Welch?s t test in (D) and (F) and Mann-Whitney U test in (C) and (E). All scale bars, 50 ?m.