Figure 4

Social behaviour in wildtype and rbfox1^{sa15940/sa15940} zebrafish after exposure to 500 µg/L fluoxetine. Zebrafish were tested in the Visually Mediated Social Preference (VMSP) and the Shoaling paradigm to assess social behaviour after being exposed to water (control group) or to 500 µg/L fluoxetine for one hour prior to testing. (A) VMSP part 1 time spent in the zone close to the first group of strangers (a1) versus the opposite area (a2), time spent freezing, and total swimming distance; (B) VMSP part 2 time spent in the zone close to the first group of strangers (a1) versus the area close to the second group of strangers (a2), time spent freezing, and total swimming distance; (C) shoaling interindividual distance, nearest neighbour distance, and total swimming distance; WT = wildtype, rbfox1^{sa15940/sa15940} = homozygous mutants, control = 0 µg/L fluoxetine, fluoxetine = 500 µg/L fluoxetine; N = 10 per group for all groups except the rbfox1^{sa15940/sa15940} control group in the VMSP (N = 9); permutation two-way ANOVA with interaction followed by post hoc permutational analysis using Benjamini and Hochberg correction except for % time in areas: Wilcoxon matched-pairs signed rank test; mean ± SD; * p/p_adj < 0.05, ** p/p_adj < 0.01.