Fig. 7
adamts9:EGFP expression in adult tissues of zebrafish. A–H, representative merged confocal images (EGFP and T-PMT channels) of heart (A), pectoral fin (B, scale bar: 200 μm), testis (C, scale bar: 200 μm; D, scale bar: 50 μm), gill (E, scale bar: 200 μm; F, scale bar: 10 μm), and kidney (G,H, scale bar: 50 μm) from a 4-month old mature male zebrafish. I, a representative gel image of RT-PCR analyses (30 cycles) of adamts9 in different tissues of adult zebrafish. J, real-time quantitative PCR (qPCR) analyses of adamts9 expression in different tissues. Results were presented as mean ± SEM (n = 6). Different letters above the error bars indicate that those groups are significantly different at P < 0.05. The expression of adamts9 in preovulatory follicles (stage IVb folic.) was omitted due to its large difference. |