FIGURE

Figure 2

ID

ZDB-FIG-240215-98

Publication

Raas et al., 2024 - Generation and characterization of a zebrafish gain-of-function ACOX1 Mitchell disease model

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Figure 2

(A) Zebrafish Acox1 protein is highly conserved compared to human ACOX1. The degree of amino acid similarity is indicated by color, with red being identical and blue being non-conserved, as visualized using PRALINE. (B) Schematic diagram of expression constructs used in zebrafish experiments. (C) Confocal z-stack images of the expression of the construct in live zebrafish larvae; immunohistochemistry for anti-GFP, and anti-RFP in the Tg(olig2:dsRed) line. Dorsal to top, rostral to the right; age 3 dpf. Note: the black line in the middle of the figure indicates where the image was stitched together. (D) Confocal z-stack images, spinal cord, rostral to the right, dorsal to the top. Expression of N237S is indicated by tagged GFP, and mature oligodendrocytes are labeled by olig2:dsRed. (E) Quantification shows no effect on oligodendrocyte numbers.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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