Fig. 3

hhex and prox1a double mutant lacks a detectable expression of molecular markers at the prospective liver site. A. WISH analysis of the molecular marker cp, bhmt, sperpina1l, and tfa in the embryos obtained from the intercrosses of male and female hhex+/− prox1a+/− double heterozygotes at 2dpf. Scale bar, 200 μm. B. WISH analysis of the liver molecular markers fabp10a and tfa in 5dpf zebrafish embryos obtained from the intercrosses of male and female hhex+/− prox1a+/− double heterozygotes. Scale bar, 200 μm. C. The bhmt post-WISH embryos were sectioned to facilitate the visualization of its expression in the liver. Green outline, intestine; yellow outline, YSL; red outlined, liver. Scale bar, 100 μm. In A-C, the number of total embryos genotyped (denominator) and the number of embryos exhibiting the displayed phenotype (numerator) are provided at the bottom right. lv, liver. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)