Fig. 6

Cebpβ promotes eosinophilopoiesis by regulating pre.Eos-related genes.

A Design of Cebpβ-target screening. RNA-Seq was performed with cebpb^+/+ and cebpb^−/− eosinophils to identify downregulated genes with cebpb mutation (470 genes, q < 0.05, baseMean > 50, log2 fold change > 0.7). ChIP-Seq was performed with Tg(hsp70:cebp1-eGFP) larvae to identify Cebpβ-binding genes (3381 genes, q < 0.05, peak fold enrich > 2, TSS ± 2 kb). B Downstream targets of Cebpβ. Seventy-five genes were found overlapped from the Cebpβ-binding genes and the downregulated genes with cebpb mutation. GO/KEGG analysis suggested that these genes are associated with ribosome (Red). The genes related to this GO term and eslec were exhibited by RNA-Seq heatmap. C Cebpβ binding on the proximal promoter of eslec. At the bottom panel, the direction of eslec transcription is marked with a black arrow, and the broad box and narrow box represent the coding sequence and UTR region, respectively. The red dotted box shows the binding peaks.