Fig. 4

Cebpb is directly suppressed by Cebp1.

A Design of Cebp1-target screening. RNA-Seq was performed with cebp1^+/+ and cebp1^−/− eosinophils to identify upregulated genes with cebp1 mutation (1,372 genes, q < 0.05, baseMean > 50, log2 fold change > 0.7). ChIP-Seq was performed with Tg(hsp70:cebp1-eGFP) larvae to identify Cebp1-binding genes (543 genes, q < 0.05, peak fold enrich > 2, TSS ± 2 kb). B GO/KEGG analysis of overlapping genes. Fifty-eight genes were found to overlap in the Cebp1-binding genes and upregulated genes of cebp1^−/− eosinophils. C Heatmap showing the top 10 highest expressed genes of all the overlapping genes. D RT-qPCR showing the relative cebpb expression in WT and cebp1^−/− eosinophils. (Student’s t test, two-sided, mean ± SEM). Three independent experiments were conducted with n = 3 in each group. E Cebp1 binding on the 3′ UTR of cebpb. At the bottom panel, the direction of cebpb transcription is marked with a black arrow, and the broad box and narrow box represent the coding sequence and UTR region, respectively. The red dotted box shows the binding peaks. F Cebp1 inhibiting the expression of cebpb. Luciferase assay demonstrated that Cebp1 inhibited the luciferase activities when the 3′UTR of cebpb was added after the luciferase gene (Luc) (Student’s t test, two-sided, mean ± SEM, ns represents no significance). Three independent experiments were conducted with n = 4 in each group. Source data are provided as a Source Data file.