FIGURE

Figure 12

ID

ZDB-FIG-240129-290

Publication

Ali et al., 2024 - Therapeutic Potential of a Novel Lytic Phage, vB_EclM_ECLFM1, against Carbapenem-Resistant Enterobacter cloacae

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Figure 12

Pathogenicity of E. cloacae and therapeutic effect of ECLFM1 observed in the zebrafish model. (A) Fish survival was compared between the LD₅₀ group and different multiplicities of infection (MOI). At MOI = 1, no significant difference in fish survival was observed compared to the LD₅₀ group; however, at MOI = 10, a statistically significant difference was seen, indicating that treatment with a higher phage MOI increased fish survival rate. Each group consisted of eight zebrafish and the experiment was performed in triplicate, and data are shown as mean ± SEM. The significance of the differences between treated and was assessed by means of the log-rank and Gehan–Breslow–Wilcoxon tests using GraphPad Prism 9. “****” indicates p < 0.0001, and “ns” means no significance. (B) In zebrafish injected with Luria-Bertani broth medium, no symptoms were observed; however, zebrafish injected with the LD₅₀ dose (3 × 10⁸ CFU/20µL) of ECL73134 exhibited swollen abdomen and superficial hemorrhage. The left, middle, and right panels show the side view, top view, and abdomen anatomy.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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