PUBLICATION
Therapeutic Potential of a Novel Lytic Phage, vB_EclM_ECLFM1, against Carbapenem-Resistant Enterobacter cloacae
- Authors
- Ali, S.F., Teh, S.H., Yang, H.H., Tsai, Y.C., Chao, H.J., Peng, S.S., Chen, S.C., Lin, L.C., Lin, N.T.
- ID
- ZDB-PUB-240123-9
- Date
- 2024
- Source
- International Journal of Molecular Sciences 25(2): (Journal)
- Registered Authors
- Keywords
- Enterobacter cloacae, Karamvirus, antimicrobial resistance, bacteriophage, multidrug resistance, phage therapy
- MeSH Terms
-
- Carbapenem-Resistant Enterobacteriaceae*
- Carbapenems/pharmacology
- Carbapenems/therapeutic use
- Enterobacter cloacae
- Bacteriophages*/genetics
- Animals
- Zebrafish
- PubMed
- 38255926 Full text @ Int. J. Mol. Sci.
Citation
Ali, S.F., Teh, S.H., Yang, H.H., Tsai, Y.C., Chao, H.J., Peng, S.S., Chen, S.C., Lin, L.C., Lin, N.T. (2024) Therapeutic Potential of a Novel Lytic Phage, vB_EclM_ECLFM1, against Carbapenem-Resistant Enterobacter cloacae. International Journal of Molecular Sciences. 25(2):.
Abstract
The global rise of multidrug-resistant Enterobacter cloacae strains, especially those that are resistant to carbapenems and produce metallo-β-lactamases, poses a critical challenge in clinical settings owing to limited treatment options. While bacteriophages show promise in treating these infections, their use is hindered by scarce resources and insufficient genomic data. In this study, we isolated ECLFM1, a novel E. cloacae phage, from sewage water using a carbapenem-resistant clinical strain as the host. ECLFM1 exhibited rapid adsorption and a 15-min latent period, with a burst size of approximately 75 PFU/infected cell. Its genome, spanning 172,036 bp, was characterized and identified as a member of Karamvirus. In therapeutic applications, owing to a high multiplicity of infection, ECLFM1 showed increased survival in zebrafish infected with E. cloacae. This study highlights ECLFM1's potential as a candidate for controlling clinical E. cloacae infections, which would help address challenges in treating multidrug-resistant strains and contribute to the development of alternative treatments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping