FIGURE

Fig. 8.

ID

ZDB-FIG-240129-251

Publication

Olson et al., 2024 - RhoA GEF Mcf2lb regulates rosette integrity during collective cell migration

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Fig. 8.

RhoA signal is lost at rosette centers in mcf2lb mutants. (A,B) Fluorescent signal from the RhoA sensor line TgBAC(cxcr4b:AHPH-GFP) at 36 hpf in WT (A) and the mcf2lb mutant (B). Tg(prim:lyn2-mCherry) marks cell membranes in the migrating pLLP. Dashed lines mark the pLLP. Arrowheads indicate rosette centers and points. Note the absence of RhoA signal at the rosette centers and points in the mcf2lb mutant pLLP. (C) Quantification of RhoA signal at the center of the trailing-most rosette (point) within the migrating pLLP in WT (n=13) and mcf2lb mutants (n=10). ***P<0.001 (unpaired two-tailed t-test). Error bars are s.d. Scale bars: 20 μm.

Expression Data

Gene:	EGFP
Fish:	sk122Tg/sk122Tg; sk85Tg/sk85Tg mcf2lb^nl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tg mcf2lb^nl26/nl26; sk122Tg/sk122Tg; sk85Tg/sk85Tg
Anatomical Term:	lateral line primordium apical part of cell
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sk122Tg/sk122Tg; sk85Tg/sk85Tg mcf2lb^nl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tg mcf2lb^nl26/nl26; sk122Tg/sk122Tg; sk85Tg/sk85Tg
Observed In:	lateral line primordium apical part of cell lateral line primordium Rho-dependent protein serine/threonine kinase activity
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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