Stmn4 mRNA could effectively rescue retinal developmental defects in both Cu stressed embryos and stmn4−/− mutants. (A) Stmn4 mRNA effectively rescued the changed expression of elavl3 and crx in retina in Cu stressed embryos (A1 − A8, A10 − A17), and the calculations of the relative expressions of elavl3 and crx in embryos from each group (A9, A18). (B) Stmn4 mRNA effectively rescued the changed expression of sox2 in stmn4−/− mutants (B1 − B8), and the calculations of the relative expressions of sox2 in embryos from each group (B9). A1 − A4, A10 − A13, B1 − B4, lateral view, anterior to the left, and dorsal to the up; A5 − A8, A14 − A17, B5 − B8, head to the up, and dorsal to the down. Scale bar, 200 μm (A1 − A8, A10 − A17, B1 − B8). ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant
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