FIGURE

Fig. 5

ID

ZDB-FIG-240110-28

Publication

Accogli et al., 2024 - Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation

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Fig. 5

WDR44 variants reduce ciliogenesis in zebrafish embryos.

Quantification (right) of ciliation in pronephric ducts (a), neuromasts (b), and olfactory placode (c) in zebrafish embryos at 3 dpf. Embryos were injected with wdr44 morpholino with or without mRNA of human WDR44 wild-type and variants, followed by staining with anti-acetylated tubulin antibody and Hoechst. Phalloidin staining was performed in (b, c). Represented immunofluorescence microscopy (IFM) (left) images. Scale bars, 10 μm. Statistical comparison with wdr44MO + WT are shown. aP = 0.0161 (D669N), 0.0002 (R733*), 0.0061 (S764F), 0.0023 (G782C), 0.0328 (H839R). bP = 0.1155 (D648G), 0.0360 (D669N), 0.0064 (R733*), 0.0003 (S764F), 0.0129 (G782C), <0.0001 (H839R). cP = 0.0309 (D669N), <0.0001 (R733*), 0.0003 (S764F), 0.0021 (G782C), P < 0.0001 (H839R). Mean ± s.e.m. from three independent experiments and total number of embryos imaged are indicated on images. Unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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