Figure 2

Gene expression in the hypothalamus of adult wildtype (A++B++) and cyp19a1b^-/- mutant (A++B–) female zebrafish in the morning (10h00) and afternoon (14h00). The abundance of mRNAs (copies/µL) for all genes measured were normalized by tata-binding protein levels within the sample and data are plotted as fold change relative to the wildtype female levels at 10h00. Parametric data were analyzed by Two-Way ANOVA tests followed by Tukey’s multiple comparisons tests and individual values are plotted with bars representing mean values (A–E, G), whilst non-parametric data were analyzed using multiple Mann-Whitney U tests with a Holm-Šídák correction for multiple comparisons and plotted as individual points with bars representing median values (F). Error bars are not displayed for clarity. Significant main effects of time (horizontal bars) and genotype (arrows) are indicated by asterisks (* p<0.05; ** p<0.01). When there is a statistically significant interaction effect, means with different letters a-b represent statistically significant differences (p<0.05).