Fig. 5

deltaA expression and AA individually predict NSC fate decisions. (A) Logistic regression model using AA and deltaA expression as covariates, showing the probability of NSC division as a function of AA for each deltaA expression status (deltaAneg MC: gray, deltaApos MC: blue). The statistical interaction between AA and deltaA was found statistically significant (type II Wald χ2 tests, P = 4.67 × 10−3). (B to C′) deltaA expression status in DC+1 pairs (symmetric deltaApos/deltaApos or asymmetric deltaAneg/deltaApos) as a function of the apical area of MCs, (B) when all MCs are considered together (deltaApos/deltaApos pair: blue, deltaAneg/deltaApos pair: orange), or (C) when deltaApos (blue) and (C′) when deltaAneg (gray) MCs are considered separately. (D) High magnification of the NSC layer showing shroom3-electroporated NSCs (Shroom3-mCherry, bottom row; control expressing mCherry only, top row) in 3-mpf Tg(deltaA:egfp) fish immunostained for GFP (cyan) and ZO1 (yellow). (E) Effect of Shroom3 overexpression on NSC AA. Shroom3-mCherry–overexpressing NSCs: n = 148, mean AA: 15 μm2; control electroporated NSCs: n = 167, mean AA: 109 μm2. Statistics: Nonparametric t test (Mann-Whitney test), P < 0.0001. (F) Effect of Shroom3 overexpression on deltaA expression. Proportion of deltaApos sNSCs among electroporated qNSCs: Shroom3-dsRed overexpression: 32% (n = 140); dsRed overexpression (control): 41% (n = 156). Statistics: Two-sided Fisher’s exact test, P value ns (not significant).